Sensitivity of anti-SARS-CoV-2 serological assays in a high-prevalence setting

Abstract Evaluation and power of seroprevalence studies depend on the performed serological assays. The aim this study was to assess four commercial tests from EUROIMMUN, DiaSorin, Abbott, Roche as well an in-house immunofluorescence neutralization test for their capability identify SARS-CoV-2 seropositive individuals in a high-prevalence setting. Therefore, 42 social working contacts German super-spreader were tested. Consistent with setting, 26 by (NT), (IFT) confirmed 23 these positive results (NT 61.9% IFT 54.8% seroprevalence). Four assays detected anti-SARS-CoV-2 antibodies 33.3-40.5% individuals. Besides overall discrepancy between NT regarding sensitivity, revealed that spike-based are better predict titer than nucleoprotein-based are.